The Journal of Cell Biology - Volume 96, issue 4

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Electronic ISSN
1540-8140
Print ISSN
0021-9525

Abstract

A procedure has been devised for isolation of triads (t- tubule/sarcoplasmic reticulum (SR) junctional complexes) from rabbit skeletal muscle. The procedure consists of preparation of a heavy microsomal fraction followed by two sequential 90-min sucrose gradient centrifugations to enrich the tria

Journal: The Journal of Cell Biology, vol. 96, no. 4, 1983

Abstract

Monoclonal antibodies against the highly conserved ubiquitous calcium- binding protein, calmodulin (CaM), were produced by immunization of mouse primary spleen cell cultures. Dissociated spleen cells were cultured for 5 d in the presence of mixed thymocyte culture conditioned media (TCM) and puri

Journal: The Journal of Cell Biology, vol. 96, no. 4, 1983

Abstract

The triad is the junctional association of transverse tubule with sarcoplasmic reticulum terminal cisternae. A procedure for the isolation of highly enriched triads from skeletal muscle has been described in the previous paper. In the present study, the structural features of isolated triads have

Journal: The Journal of Cell Biology, vol. 96, no. 4, 1983