Nucleic Acids Research - Volume 36, issue 5

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Electronic ISSN
1362-4962
Print ISSN
0305-1048

Abstract

Many in vitro and in vivo applications for transgenesis require co-expression of heterologous genes. The use of internal ribosome entry sites (IRESs) in dicistronic expression vectors enables the expression of two genes controlled by one promoter in target cells or whole organisms.

Journal: Nucleic Acids Research, vol. 36, no. 5, 2008

Abstract

Locked nucleic acid (LNA) is a chemically modified nucleic acid with its sugar ring locked in an RNA-like (C3′-endo) conformation. LNAs show extraordinary thermal stabilities when hybridized with DNA, RNA or LNA itself. We performed molecular dynamics simulations on five isosequential duplexes (L

Journal: Nucleic Acids Research, vol. 36, no. 5, 2008