Nucleic Acids Research - Volume 36, issue 12

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Electronic ISSN
1362-4962
Print ISSN
0305-1048

Abstract

The restriction endonuclease EcoRV can rapidly locate a short recognition site within long non-cognate DNA using ‘facilitated diffusion’. This process has long been attributed to a sliding mechanism, in which the enzyme first binds to the DNA via nonspecific interaction and then moves along the D

Journal: Nucleic Acids Research, vol. 36, no. 12, 2008

Abstract

Although frequently used as protein production host, there is only a limited set of promoters available to drive the expression of recombinant proteins in Pichia pastoris. Fine-tuning of gene expression is often needed to maximize product yield and quality. However, for efficient knowledge

Journal: Nucleic Acids Research, vol. 36, no. 12, 2008